Archive for the ‘Fenugreek Extract (4 – Hydroxyisoleucine)’ Category

Fenugreek Extract (4 – Hydroxyisoleucine)

Introduction :
4 – Hydroxyisoleucine is an amino acid extracted from the Fenugreek seeds which is devoid of characteristic smell and bitter taste that of fenugreek seeds and leaves. These natural active chemicals extracted from Fenugreek seed is highly unstabilised and once exposed to air and moisture starts to degrade. Hence it is stabilised for long shelf life and packed under Nitrogen atmosphere. Numerous animal studies and preliminary trials in humans have found that fenugreek seed extract called 4-Hydroxyisolucein can help support healthy blood sugar and serum cholesterol levels in people with diabetes. 4-Hydroxyisoleucine is now widely used in the nutraceutical industries as an insulinotropic & anti diabetic compound.

Extraction Process :
A study was made of the enzyme in fenugreek implicated in the biosynthesis of 4-hydroxyisoleucine, which is an unusual amino acid known for its insulin stimulating effect. 4-hydroxyisoleucine was detected by HPLC following isoleucine incubation with a cell-free extract from etiolated 6-day old fenugreek seedlings in the presence of various cofactors. The reaction showed that 4-hydroxyisoleucine formation is dependent on the presence of Fe2+, 2-oxoglutarate, ascorbate, and oxygen. This suggests that a 2-oxoacid-dependent dioxygenase plays a key role in this biosynthetic pathway.

Active Principle :

  1. 4-hydroxyisoleucine in Fenugreek extract plays a valuable role in insulin promotion and glucose regulation, which may help to reduce body fat.

  2. 4-hydroxyisoleucine has a nutrient partitioning effect, which means it may help to shuttle nutrients to muscle cells preferentially over fat cells.

  3. 4-hydroxyisoleucine appears to be glucose dependent. The higher the blood glucose level is, the greater the insulin promoting response elicited by 4-hydroxyisoleucine. By helping to regulate insulin needs, 4-hydroxyisoleucine works as an adaptogen.

Bulk Fenugreek Seeds

Uses of Fenugreek Extract :

  1. Research has shown that 4 – Hydroxyisoleucine helps lower blood sugar in diabetics.

  2. 4 – Hydroxyisoleucine indirectly helps to lower the levels of triglycerides.

  3. 4 – Hydroxyisoleucine indirectly helps reduce weight.

Other Uses :
The fenugreek seed extract is also used commercially in artificial flavorings such as vanilla, caramel, butterscotch and especially maple syrup.

4 – Hydroxyisoleucine :
Branched chain amino acid found in the seeds of Fenugreek

  • (2S, 3R, 4S) 4 – Hydroxyisoleucine is a major isomer.

  • (2R, 3R, 4S) 4 – Hydroxyisoleucine is a minor isomer.

Other compounds

  • Lactones.

  • Other Amino acids like Isoleucine, Leucine and Argenine.

Importance points to Note :

  • If separation and measurement of 4-Hydroxyisoleucine is not good by HPLC, total amino acids get reported as 4-Hydroxyisoleucine.

  • 4-Hydroxyisoleucine gets oxidized to 2-Keto-3Methyl-4Hydroxy Valeric Acid in contact with air, water, heat and light with characteristic strong odor.

  • Stabilization is very important for self-life.

  • Due to instability the material develops a very strong odor due to formation of oxidized product.

  • Characteristic strong odor is the way to identify this material.

  • Bitterness is caused by the presence of Saponins from fenugreek seed.

We can offer :

  • 40% Assay 4-Hydroxyisoleucine.

  • 20% Assay 4-Hydroxyisoleucine.

These are natural products extracted from Fenugreek seed and packed under Nitrogen Atmosphere.

Once opened it degrades in contact with air and moisture.

We can also offer “stabilized deodorized and debitterised.”

  • 14% Assay 4-Hydroxyisoleucine.

  • 20% Assay 4-Hydroxyisoleucine.

This does not have characteristic smell and bitter taste. This is stabilized for long self-life.

Highlights of Our Product :

  • ISO 9000 certified manufacturing unit.
  • Organic raw material through dedicated farmers and supplier of raw-material.
  • cGMP, manufacturing and Good Lab Practice.
  • Quality specification, with microbial level less than 300 CFU/g and free from yeast, mold and other contaminants.
  • Free from any heavy metal Content.
  • Only available in Bulk (minimum quantity 100 Kg)
  • Customized and contract manufacturing as per customer requirement.
  • UNTUK PEMESANAN HUBUNGI BIN MUHSIN HP: 085227044550 Tlp: 021-91913103 SMS ONLY: 081213143797
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